مؤسسة الشرق الأوسط للنشر العلمي
عادةً ما يتم الرد في غضون خمس دقائق
The liver-metabolic axis plays key roles in systemic homeostasis and integrates hepatic function and hormonal regulation Oxidative stress can interfere with this balance, leading to poor insulin signaling and decreased leptin effectiveness, causing metabolic imbalance. This study was conducted to determine the long-term effects of oral glutathione on liver enzymes and some metabolic hormones, using female rats as subjects. Eighty adult female Wistar rats weighing between 200 and 250 grams were randomized into two equal halves. For a period of 60 consecutive days, from December 1, 2025, to February 1, 2026, the control group received physiological saline daily while treatment group was provided oral glutathione with a dose equivalent to 500 mg per kilogram per day. Serum alanine aminotransferase and aspartate aminotransferase (ALT and AST) was carried out using kinetic colorimetric techniques. We assessed insulin and leptin levels by using ELISA kits. Supplementing with glutathione had a major impact on the reduction of serum alanine aminotransferase which was 35.40 ± 2.28 U/L as against 48.62 ± 3.15 U/L in the control group. Likewise, the levels of aspartate aminotransferase in the treated rats (87.12 ±5.92 U/L) were found to decreased as compared to the controls (112.45±7.80 U/L) at probability values <0.05. These alterations signify a greater stability of the membranes of hepatocytes. Moreover, compared to controls (8.45 ± 0.72 µIU/mL; P< 0.01), the mean serum insulin level of glutathione treated group was lower (6.12 ± 0.45 µIU/mL). Leptin concentrations of the treatment group were also significantly decreased to 2.30 ± 0.15 ng/mL compared to controls of 3.85 ± 0.28 ng/mL (p < 0.001), demonstrating better leptin responsiveness. During the duration of the study, we observed no negative clinical signs or body weight changes. The study concludes that oral glutathione long-term supplementation was able to stabilize the liver-metabolic axis in females. This effect seems to be caused by the protection of liver function and enhancement of insulin and leptin signals. Glutathione thus appears to be an interesting candidate for treating metabolic disorders and improving hormonal imbalance under oxidative stress conditions.