مؤسسة الشرق الأوسط للنشر العلمي
عادةً ما يتم الرد في غضون خمس دقائق
Gastritis is an Inflammation of the lining of the stomach. The inflammation of gastritis is most often the result of infection with the same bacterium that causes most stomach ulcers or the regular use of certain pain relievers. Drinking too much alcohol also can contribute to gastritis. This study aims to estimate the levels of immunological markers Interleukin-8 (IL-8) and Interleukin-6 (IL-6), and to analyze the expression of specific genes( ClpB and BspA) in patients with gastritis. By comparing these markers and gene expressions in gastritis patients with healthy controls, we seek to better understand the immunological and genetic factors involved in the disease process. Objectives:To estimate the levels of IL-8 and IL-6 in the gingival crevicular fluid (GCF) and serum of gastritis patients.To analyze the expression of genes related to inflammation and tissue degradation in the gastritis tissues of gastritis patients.To compare these markers and gene expressions between gastritis patients and healthy controls.To investigate the correlation between cytokine levels, gene expression, and clinical parameters of gastritis. Materials and Methods: This study was carried out in Hilla Teaching Hospital in Babylon from (November 2023 to March 2024), patients with gastritis (60 patients 55 male and 5 female) with range age (25-60) years.Sample Collection(Gingival crevicular fluid (GCF) samples from gastritis patients .Blood samples for serum analysis. gastritis tissue samples for gene expression analysis.Quantification of IL-8 and IL-6 levels in GCF and serum using ELISA (Enzyme-Linked Immunosorbent Assay).quantitative real-time PCR (qRT-PCR) for analyzing the expression of target genes.Results: In this study, 120 samples were detected for present of Helicobacter pylori by q RT-PCR technique, the specific ClpB and BspA primers genes were used for the amplification of a fragment these genes for identification of Helicobacter pylori .Conclusion : The study highlight the potential of using immunological markers and gene expression profiles as diagnostic tools for early detection and progression monitoring of gastritis. Furthermore, understanding the molecular mechanisms underlying these changes can inform the development of targeted therapeutic strategies aimed at modulating the immune response, promoting tissue regeneration, and ultimately improving gastritis health outcomes.Future research should focus on longitudinal studies to validate these biomarkers and explore their utility in clinical practice. Integrating molecular data with clinical parameters will enhance our ability to personalize treatment approaches and improve prognosis for patients suffering from gastritis